Journal: eBioMedicine
Article Title: 5-Hydroxymethylcytosine profiles in circulating cell-free DNA serve as potential biomarkers for diagnosis and classification of adenomyosis
doi: 10.1016/j.ebiom.2026.106225
Figure Lengend Snippet: Role of HOXD9 in APP Overexpression-Mediated Cell Migration. (a). Transwell migration experiment revealed the difference of cell migration ability in the control group (NC group), the APP overexpression group ( OE-APP group) and the APP overexpression group simultaneously knocked down HOXD9 group, with A scale of 100 micron. (b). The number of migrating cells in the OE-APP group was significantly higher than that in the NC group (p < 0.0001), and the number of migrating cells in the OE-APP + si-HOXD9 group was significantly lower than that in the OE-APP group (p < 0.01). (c). Representative phase-contrast images of the scratch-wound assay at 0, 12 and 24 h after wounding (scale bar = 200 μm). A confluent monolayer of the indicated groups (NC, OE-APP, OE-APP + si-HOXD9) was wounded with a sterile 200 μL pipette tip, washed three times with PBS to remove debris and incubated in serum-free medium at 37 °C, 5% CO 2 . Wound width was measured in triplicate wells using ImageJ and expressed as percentage closure relative to 0 h. Quantification (right panel) shows mean ± SD from three independent experiments. Two-way repeated-measures ANOVA followed by Bonferroni post-test: ∗∗p < 0.01, ∗∗∗∗p < 0.0001 versus NC at the same time point.
Article Snippet: The clarified plasma was immediately stored at −80 °C.Plasma cfDNA was extracted using the Quick-cfDNA Serum & Plasma Kit (ZYMO, Cat# D4076) and quantified with a Qubit 3.0 fluorometer (Thermo, Cat# Q33216 ).
Techniques: Over Expression, Migration, Control, Scratch Wound Assay Assay, Sterility, Transferring, Incubation